| Gram Positive Bacteria | Gram Negative Bacteria |
| 1. Cell wall thicker contains only traces of lipids | 1. Cell wall thinner may contain upto 20% lipids |
| 2. Striking simplicity of amino acids | 2. All the amino acids are present |
| 3. Muramic acid more | 3. Muramic acid is less |
| 4. Teichoic acid present | 4. Teichoic acid absent |
| 5. Glutamic acid and alanine occur in large amounts | 5. Glutamic acid and alanine occur in small amounts |
| 6. Diaminopimelic acid in some | 6. Diaminopimelic acid in large |